diff --git a/app/controllers/api/v1_alpha/collection_items_controller.rb b/app/controllers/api/v1_alpha/collection_items_controller.rb
index cc2e5cdef1..21699a5b6f 100644
--- a/app/controllers/api/v1_alpha/collection_items_controller.rb
+++ b/app/controllers/api/v1_alpha/collection_items_controller.rb
@@ -11,6 +11,7 @@ class Api::V1Alpha::CollectionItemsController < Api::BaseController
 
   before_action :set_collection
   before_action :set_account, only: [:create]
+  before_action :set_collection_item, only: [:destroy]
 
   after_action :verify_authorized
 
@@ -23,6 +24,14 @@ class Api::V1Alpha::CollectionItemsController < Api::BaseController
     render json: @item, serializer: REST::CollectionItemSerializer
   end
 
+  def destroy
+    authorize @collection, :update?
+
+    @collection_item.destroy
+
+    head 200
+  end
+
   private
 
   def set_collection
@@ -35,6 +44,10 @@ class Api::V1Alpha::CollectionItemsController < Api::BaseController
     @account = Account.find(params[:account_id])
   end
 
+  def set_collection_item
+    @collection_item = @collection.collection_items.find(params[:id])
+  end
+
   def check_feature_enabled
     raise ActionController::RoutingError unless Mastodon::Feature.collections_enabled?
   end
diff --git a/config/routes/api.rb b/config/routes/api.rb
index 563191614c..3fa1aa7af2 100644
--- a/config/routes/api.rb
+++ b/config/routes/api.rb
@@ -13,7 +13,7 @@ namespace :api, format: false do
     resources :async_refreshes, only: :show
 
     resources :collections, only: [:show, :create, :update, :destroy] do
-      resources :items, only: [:create], controller: 'collection_items'
+      resources :items, only: [:create, :destroy], controller: 'collection_items'
     end
   end
 
diff --git a/spec/requests/api/v1_alpha/collection_items_spec.rb b/spec/requests/api/v1_alpha/collection_items_spec.rb
index 880fd5d47d..5c44a7edf8 100644
--- a/spec/requests/api/v1_alpha/collection_items_spec.rb
+++ b/spec/requests/api/v1_alpha/collection_items_spec.rb
@@ -52,4 +52,53 @@ RSpec.describe 'Api::V1Alpha::CollectionItems', feature: :collections do
       end
     end
   end
+
+  describe 'DELETE /api/v1_alpha/collections/:collection_id/items/:id' do
+    subject do
+      delete "/api/v1_alpha/collections/#{collection.id}/items/#{item.id}", headers: headers
+    end
+
+    let(:collection) { Fabricate(:collection, account: user.account) }
+    let(:item) { Fabricate(:collection_item, collection:) }
+
+    it_behaves_like 'forbidden for wrong scope', 'read'
+
+    context 'when user is owner of the collection' do
+      context 'when item belongs to collection' do
+        it 'deletes the collection item and returns http success' do
+          item # Make sure this exists before calling the API
+
+          expect do
+            subject
+          end.to change(collection.collection_items, :count).by(-1)
+
+          expect(response).to have_http_status(200)
+        end
+      end
+
+      context 'when item does not belong to to collection' do
+        let(:item) { Fabricate(:collection_item) }
+
+        it 'returns http not found' do
+          item # Make sure this exists before calling the API
+
+          expect do
+            subject
+          end.to_not change(CollectionItem, :count)
+
+          expect(response).to have_http_status(404)
+        end
+      end
+    end
+
+    context 'when user is not the owner of the collection' do
+      let(:collection) { Fabricate(:collection) }
+
+      it 'returns http forbidden' do
+        subject
+
+        expect(response).to have_http_status(403)
+      end
+    end
+  end
 end